Review



complete l1cam  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology complete l1cam
    Fig. 6 Nuclear <t>L1CAM</t> localisation with vascular EndMT and γ-H2AX L1CAM colocalization in heart tissues from patients with cardiomyopathy. a Immunofluorescence staining of L1CAM and VE-cadherin in heart tissues from patients with cardiomyopathy (n = 12) and patients without cardiomyopathy (n = 8) (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p = 0.0004). b Immunofluorescence staining of γ-H2AX and CD31 in heart tissues from patients with cardiomyopathy and patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (Normal vs. γ-H2AX+ CD31+ p > 0.0001; γ-H2AX‒ CD31+ vs. γ-H2AX+ CD31+ p = 0.0003) c Immunofluorescence staining of L1CAM, α-SMA, and CD31 in heart tissues from patients with cardiomyopathy and from patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among α-SMA-CD31+ and α-SMA+CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p > 0.0001). (a two-tailed student’s t-test; b, c one-way ANOVA for multiple comparisons).
    Complete L1cam, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+l1cam/NCAM-L1+Antibody/pm34078883-408-39-44
    Average 94 stars, based on 78 article reviews
    complete l1cam - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage."

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage.

    Journal: Nature communications

    doi: 10.1038/s41467-021-23478-1

    Fig. 6 Nuclear L1CAM localisation with vascular EndMT and γ-H2AX L1CAM colocalization in heart tissues from patients with cardiomyopathy. a Immunofluorescence staining of L1CAM and VE-cadherin in heart tissues from patients with cardiomyopathy (n = 12) and patients without cardiomyopathy (n = 8) (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p = 0.0004). b Immunofluorescence staining of γ-H2AX and CD31 in heart tissues from patients with cardiomyopathy and patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (Normal vs. γ-H2AX+ CD31+ p > 0.0001; γ-H2AX‒ CD31+ vs. γ-H2AX+ CD31+ p = 0.0003) c Immunofluorescence staining of L1CAM, α-SMA, and CD31 in heart tissues from patients with cardiomyopathy and from patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among α-SMA-CD31+ and α-SMA+CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p > 0.0001). (a two-tailed student’s t-test; b, c one-way ANOVA for multiple comparisons).
    Figure Legend Snippet: Fig. 6 Nuclear L1CAM localisation with vascular EndMT and γ-H2AX L1CAM colocalization in heart tissues from patients with cardiomyopathy. a Immunofluorescence staining of L1CAM and VE-cadherin in heart tissues from patients with cardiomyopathy (n = 12) and patients without cardiomyopathy (n = 8) (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p = 0.0004). b Immunofluorescence staining of γ-H2AX and CD31 in heart tissues from patients with cardiomyopathy and patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (Normal vs. γ-H2AX+ CD31+ p > 0.0001; γ-H2AX‒ CD31+ vs. γ-H2AX+ CD31+ p = 0.0003) c Immunofluorescence staining of L1CAM, α-SMA, and CD31 in heart tissues from patients with cardiomyopathy and from patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among α-SMA-CD31+ and α-SMA+CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p > 0.0001). (a two-tailed student’s t-test; b, c one-way ANOVA for multiple comparisons).

    Techniques Used: Staining, Two Tailed Test

    Related Articles

    Western Blot:

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
    Article Snippet: At least five images per section were acquired for quantification, and the positively stained areas were evaluated using ImageJ software 1.49 v. ( http://imagej.net/ ). .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1-CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc-47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc-10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc-135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage.
    Article Snippet: .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 NATURE COMMUNICATIONS | (2021) 12:3279 | https://doi.org/10.1038/s41467-021-23478-1 | www.nature.com/naturecommunications 15 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Immunohistochemistry:

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
    Article Snippet: At least five images per section were acquired for quantification, and the positively stained areas were evaluated using ImageJ software 1.49 v. ( http://imagej.net/ ). .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1-CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc-47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc-10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc-135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage.
    Article Snippet: .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 NATURE COMMUNICATIONS | (2021) 12:3279 | https://doi.org/10.1038/s41467-021-23478-1 | www.nature.com/naturecommunications 15 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Immunofluorescence:

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
    Article Snippet: At least five images per section were acquired for quantification, and the positively stained areas were evaluated using ImageJ software 1.49 v. ( http://imagej.net/ ). .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1-CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc-47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc-10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc-135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage.
    Article Snippet: .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 NATURE COMMUNICATIONS | (2021) 12:3279 | https://doi.org/10.1038/s41467-021-23478-1 | www.nature.com/naturecommunications 15 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Staining:

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
    Article Snippet: At least five images per section were acquired for quantification, and the positively stained areas were evaluated using ImageJ software 1.49 v. ( http://imagej.net/ ). .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1-CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc-47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc-10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc-135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage.
    Article Snippet: .. Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 NATURE COMMUNICATIONS | (2021) 12:3279 | https://doi.org/10.1038/s41467-021-23478-1 | www.nature.com/naturecommunications 15 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc135886; Santa Cruz Biotechnology). .. To visualise actin stress fibres, cells were stained with Alexa Fluor 488-conjugated phalloidin (1:40; Invitrogen), which specifically binds polymerised F-actin.



    Similar Products

    complete l1cam  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology complete l1cam
    Fig. 6 Nuclear <t>L1CAM</t> localisation with vascular EndMT and γ-H2AX L1CAM colocalization in heart tissues from patients with cardiomyopathy. a Immunofluorescence staining of L1CAM and VE-cadherin in heart tissues from patients with cardiomyopathy (n = 12) and patients without cardiomyopathy (n = 8) (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p = 0.0004). b Immunofluorescence staining of γ-H2AX and CD31 in heart tissues from patients with cardiomyopathy and patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (Normal vs. γ-H2AX+ CD31+ p > 0.0001; γ-H2AX‒ CD31+ vs. γ-H2AX+ CD31+ p = 0.0003) c Immunofluorescence staining of L1CAM, α-SMA, and CD31 in heart tissues from patients with cardiomyopathy and from patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among α-SMA-CD31+ and α-SMA+CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p > 0.0001). (a two-tailed student’s t-test; b, c one-way ANOVA for multiple comparisons).
    Complete L1cam, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+l1cam/NCAM-L1+Antibody/pm34078883-408-39-44
    Average 94 stars, based on 1 article reviews
    complete l1cam - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 6 Nuclear L1CAM localisation with vascular EndMT and γ-H2AX L1CAM colocalization in heart tissues from patients with cardiomyopathy. a Immunofluorescence staining of L1CAM and VE-cadherin in heart tissues from patients with cardiomyopathy (n = 12) and patients without cardiomyopathy (n = 8) (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p = 0.0004). b Immunofluorescence staining of γ-H2AX and CD31 in heart tissues from patients with cardiomyopathy and patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (Normal vs. γ-H2AX+ CD31+ p > 0.0001; γ-H2AX‒ CD31+ vs. γ-H2AX+ CD31+ p = 0.0003) c Immunofluorescence staining of L1CAM, α-SMA, and CD31 in heart tissues from patients with cardiomyopathy and from patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among α-SMA-CD31+ and α-SMA+CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p > 0.0001). (a two-tailed student’s t-test; b, c one-way ANOVA for multiple comparisons).

    Journal: Nature communications

    Article Title: An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage.

    doi: 10.1038/s41467-021-23478-1

    Figure Lengend Snippet: Fig. 6 Nuclear L1CAM localisation with vascular EndMT and γ-H2AX L1CAM colocalization in heart tissues from patients with cardiomyopathy. a Immunofluorescence staining of L1CAM and VE-cadherin in heart tissues from patients with cardiomyopathy (n = 12) and patients without cardiomyopathy (n = 8) (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p = 0.0004). b Immunofluorescence staining of γ-H2AX and CD31 in heart tissues from patients with cardiomyopathy and patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (Normal vs. γ-H2AX+ CD31+ p > 0.0001; γ-H2AX‒ CD31+ vs. γ-H2AX+ CD31+ p = 0.0003) c Immunofluorescence staining of L1CAM, α-SMA, and CD31 in heart tissues from patients with cardiomyopathy and from patients without cardiomyopathy (upper panels) and quantification (lower panel) of nuclear L1CAM+ cells among α-SMA-CD31+ and α-SMA+CD31+ cells (magnification, ×400). Scale bar = 10 μm (enlarged, 2 μm). Error bars represent mean ± SEM (p > 0.0001). (a two-tailed student’s t-test; b, c one-way ANOVA for multiple comparisons).

    Article Snippet: Immunoblotting, immunohistochemistry, and immunofluorescence staining were performed using primary antibodies against the N-terminal domain of L1CAM (immunohistochemistry/immunofluorescence 1:100; sc-31032; Santa Cruz Biotechnology), L1CT (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; LS-B9803; LSBio; immunofluorescence 1:200; sc-53386; Santa Cruz Biotechnology; immunofluorescence 1:200; ab123990; Abcam), complete L1CAM (immunoblotting 1:1000; sc-53386; Santa Cruz Biotechnology), γ-H2AX (immunohistochemistry/immunofluorescence 1:500, immunoblotting 1:1000; 05-636; Millipore), CD31 NATURE COMMUNICATIONS | (2021) 12:3279 | https://doi.org/10.1038/s41467-021-23478-1 | www.nature.com/naturecommunications 15 (immunohistochemistry/immunofluorescence 1:200; #AF3628; R&D Systems; immunoblotting 1:1000; 28364; Abcam), GAPDH (immunoblotting 1:1000; sc47724; Santa Cruz Biotechnology), lamin B (immunoblotting 1:1000; sc-6216; Santa Cruz Biotechnology), αSMA (1:2000; A5228; Sigma-Aldrich), VCAM-1 (immunoblotting 1:1000; sc-1504; Santa Cruz Biotechnology), p-ATM (immunofluorescence 1:200; 05-740; Millipore), 53BP1 (immunofluorescence 1:200; sc10914; Santa Cruz Biotechnology), and DNA-PKcs (immunofluorescence 1:200; sc135886; Santa Cruz Biotechnology).

    Techniques: Staining, Two Tailed Test